Traditional blood collection for whole blood gene expression profiling and biomarker analysis often requires a visit to a healthcare professional, posing challenges for special populations (sick, fragile health, immobile), or for large-scale studies requiring repeated sampling. Blood microsampling, the process of obtaining small volumes of capillary blood, holds great promise to overcome these challenges. We have developed a cost-effective, high-throughput platform for processing self-collected blood microsamples using Mitra Volumetric Adsorptive Microsampling (VAMS) devices for downstream RNA sequencing.