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Steve Lefever
PostDoctoral Fellow (01/2008-09/2020)
Ghent University
Interests
Cancer
Non-coding RNA
Pediatric tumors
Bioinformatics
Latest
mRNA capture sequencing and RT-qPCR for the detection of pathognomonic, novel, and secondary fusion transcripts in FFPE tissue: a sarcoma showcase
Validation of Circular RNAs Using RT‐qPCR After Effective Removal of Linear RNAs by Ribonuclease R
The RNA Atlas expands the catalog of human non-coding RNAs
Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas
circRNA
Charting extracellular transcriptomes in The Human Biofluid RNA Atlas
The RNA Atlas, a single nucleotide resolution map of the human transcriptome
LncRNAs as novel players in neuroblastoma tumor biology
Integrative analysis identifies lincRNAs up- and downstream of neuroblastoma driver genes
Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR
A mechanistic classification of clinical phenotypes in neuroblastoma
Long noncoding RNA expression profiling in cancer: Challenges and opportunities
Targeted Genomic Screen Reveals Focal Long Non-Coding RNA Copy Number Alterations in Cancer Cell Lines
A high-throughput 3’ UTR reporter screening identifies microRNA interactomes of cancer genes
The mutational landscape of <i>MYCN, Lin28b</i> and <i>ALK<sup>F1174L</sup></i> driven murine neuroblastoma mimics human disease
The mutational landscape of MYCN, Lin28b and ALKF1174L driven murine neuroblastoma mimics human disease
High-throughput PCR assay design for targeted resequencing using primerXL
decodeRNA— predicting non-coding RNA functions using guilt-by-association
Erratum: Corrigendum: Differential expression of lncRNAs during the HIV replication cycle: an underestimated layer in the HIV-host interplay
Differential expression of lncRNAs during the HIV replication cycle: an underestimated layer in the HIV-host interplay
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
miRBase Tracker
primerXL
RDML database
Targeted resequencing and variant validation using pxlence PCR assays
RDML-Ninja and RDMLdb for standardized exchange of qPCR data
Flexible, Scalable, and Efficient Targeted Resequencing on a Benchtop Sequencer for Variant Detection in Clinical Practice
Expressed Repeat Elements Improve RT-qPCR Normalization across a Wide Range of Zebrafish Gene Expression Studies
miRBase Tracker: keeping track of microRNA annotation changes
Target enrichment using parallel nanoliter quantitative PCR amplification
Single-Nucleotide Polymorphisms and Other Mismatches Reduce Performance of Quantitative PCR Assays
Regulatory microRNA Network Identification in Bovine Blastocyst Development
Focal DNA Copy Number Changes in Neuroblastoma Target MYCN Regulated Genes
Molecular diagnostics for congenital hearing loss including 15 deafness genes using a next generation sequencing platform
Development of bioinformatics tools for high-throughput PCR applications
Massively parallel sequencing for early molecular diagnosis in Leber congenital amaurosis
Practical Tools to Implement Massive Parallel Pyrosequencing of PCR Products in Next Generation Molecular Diagnostics
The microRNA body map: dissecting microRNA function through integrative genomics
Measurable impact of RNA quality on gene expression results from quantitative PCR
Analysing 454 amplicon resequencing experiments using the modular and database oriented Variant Identification Pipeline
RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
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