The RNA Atlas, a single nucleotide resolution map of the human transcriptome

Abstract

The human transcriptome consists of various RNA biotypes including multiple types of non-coding RNAs (ncRNAs). Current ncRNA compendia remain incomplete partially because they are almost exclusively derived from the interrogation of small- and polyadenylated RNAs. Here, we present a more comprehensive atlas of the human transcriptome that is derived from matching polyA-, total-, and small-RNA profiles of a heterogeneous collection of nearly 300 human tissues and cell lines. We report on thousands of novel RNA species across all major RNA biotypes, including a hitherto poorly-cataloged class of non-polyadenylated single-exon long non-coding RNAs. In addition, we exploit intron abundance estimates from total RNA-sequencing to test and verify functional regulation by novel non-coding RNAs. Our study represents a substantial expansion of the current catalogue of human ncRNAs and their regulatory interactions. All data, analyses, and results are available in the R2 web portal and serve as a basis to further explore RNA biology and function.

Publication
bioRxiv
Lucia Lorenzi
Lucia Lorenzi
Doctoral Fellow (09/2016-02/2021)
Francisco Avila Cobos
Francisco Avila Cobos
PostDoctoral Fellow (10/2014-02/2022)

the “deconvolution” guy

Pieter-Jan Volders
Pieter-Jan Volders
PostDoctoral Fellow (07/2011-02/2022)

LncRNA aficionado working with transcriptomics and proteomics

Justine Nuytens
Justine Nuytens
Lab Technician
Katrien Vanderheyden
Katrien Vanderheyden
Lab Technician
Jasper Anckaert
Jasper Anckaert
Bioinformatician

The real Jasper

Steve Lefever
Steve Lefever
PostDoctoral Fellow (01/2008-09/2020)
Jo Vandesompele
Jo Vandesompele
Professor

RNA addict trying to connect all the dots

Pieter Mestdagh
Pieter Mestdagh
Professor

Studying non-coding RNAs in cancer.