BACKGROUND: High-risk neuroblastoma tumors respond differently to therapy due to their unique molecular makeup and tumor microenvironment. Therefore, molecularly profiling of these tumors helps tailoring treatment. Spatial transcriptomics enables detailed transcriptional profiling while preserving spatial context. We are working on a customized version of this technology using a DNA microarray printer and an in-house developed library preparation protocol.
AIMS: We aim to develop a novel, cost-effective spatial transcriptomics platform, providing an unbiased transcriptome view at single-cell resolution.